G418 and minimal medium!

Aidan Weatherill Aweather at worf.molbiol.ox.ac.uk
Fri Sep 11 10:16:18 EST 1998


Haico van Attikum wrote in message
<35F8EB3C.5ED84F42 at rulbim.leidenuniv.nl>...
>Hello,
>
>I want to select for G418 resistant yeast strains on minimal medium.
>Does anybody know if this works, because in literature I found that
>selection was only performed using  rich medium.
>
>With kind regards,
>
>Haico

G418 selection only works rich media, I dont know why this is but thats the
way of the world.
Sorry.





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