ribosomal RNA and northerns

[Christopher M. Hammell]

Hello,
    I have a mRNA that runs with the same mobility as 28S rRNA. The
ribosomal RNA causes my message to run awkwardly and results in a
smeary banding patterns when I probe it for northerns.  I don't want to
poly A+ select because in my mutant strains, polyadenylation is
inefficient at the non-permissive temperature.

Any help would be appreciated,

Chris

C.M. Hammell
hammell at dartmouth.edu
Dartmouth Col.
Hanover NH 03755
(603)650-1905