ribosomal RNA and northerns

d.jansma at utoronto.ca d.jansma at utoronto.ca
Tue Sep 15 06:43:57 EST 1998


Could you do a primer extension or RNase protection to quantify your message?

>In article <8396847 at dasher.Dartmouth.EDU>,
>Christopher.M.Hammell at Dartmouth.EDU (Christopher M. Hammell) wrote:
>
>>Hello,
>>    I have a mRNA that runs with the same mobility as 28S rRNA. The
>>ribosomal RNA causes my message to run awkwardly and results in a
>>smeary banding patterns when I probe it for northerns.  I don't want to
>>poly A+ select because in my mutant strains, polyadenylation is
>>inefficient at the non-permissive temperature.





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