Yeast Transformation

[Jacques Paysan]

Dear Yeasters,

It is my understanding, without being a yeast expert, that yeast cells are
most suitable for transformation in their log growth phase.  Can anyone
explain to me the rational arguments for this fact?

Furthermore, is there any rule for the ratio between DNA amount, carrier
DNA amount, and absolute number of log-phase yeast cells to be used in an
optimal transformation setup?

Any suggestions directed to the (background-interested) two-hybrid user
will be greatly appreciated.

Best regards,
Jacques

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Dr. Jacques Paysan
Universitaet Tuebingen
Institut fuer Physiologie, Abt. II
Ob dem Himmelreich 7
D-72074 Tuebingen
Germany

Telefon: (07071) 29 771 73
Telefax: (07071) 87 815