protein-protein crosslinking

[Scott-Navigator]

Dear Louise:

I believe that Charlie Barlowe used this reagent to crosslink Emp24p and Erv25p
in lysates.  This was described in the JBC 1996.  Look for Belden as the first
author.

Good luck,
Scott Moye-Rowley

Louise.Chang at mcmail.vanderbilt.edu wrote:

> Is anyone familiar with a protocol/reference for a reversible protein-protein
> crosslinker (e.g. DSP) in intact yeast cells (S. pombe) or in a crude yeast
> extract?
>
> Thank you
> Louise Chang
>
> louise.chang at mcmail.vanderbilt.edu