mutagenesis of cloned yeast gene

[Petra B. Ross-MacDonald]

You should check out mini-Tn mutagenesis

ycmi/med/yale/edu/YGAC/home.html

The method may not be the best way to get subtle variant alleles,
although it has been used to generate ts alleles. But if you want to
localize your protein, mTn tagging is a great way to go.



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Petra Ross-Macdonald, Ph.D
Senior Research Investigator
Bristol Myers Squibb PRI, Hopewell
PO Box 5400
Princeton NJ 08543-5400

Phone (609) 818 7118
Fax (609) 818-6058
rossmacp at bms.com