PCR mediated gene disruption vs. integrating vector

Björn Johansson bjorn.johansson at tmb.lth.se
Mon Jan 11 09:23:36 EST 1999


Hello Yeast People,

I have a question regarding the integration fequency of PCR mediated 
gene integration compared to an integrating vector using one of the 
common markers.

The regions of homology whith the PCR method is only 
30-45bp on each side, an integrating vector can have very long regions 
of homology with the site of recombination. 

Is the frequency of integration much higher for the integrating vector 
than the PCR method ?

Thank you in advance for any answer !

Sincerely,
Björn Johansson






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