G418, Yeast genetic background difference? Others?

Jonathan Katz jonathan at ucla.edu
Wed Mar 24 17:00:35 EST 1999


Krasen Dimitrov wrote:
> 
> I appologize if this sounds obnoxiuos, but are you sure you didn't knock
> something important in your diploid with the Kan marker?
>

[replying for Hui]...   Not obnoxious at all...  The gene of interest
was also knocked out in a haploid strain which subsequently grows
fine...  
 
> Hui Cai wrote:
> 
> > Hello All.
> >   I had a couple of quick questions, please, if anybody could help out.
> >
> > 1)  Is there a genetic background effect of G418 resistance using the
> > NeoR gene?
> > (I have a diploid strain with the resistance that grows fine on
> > YPD+G418, but,
> > after I sporulate none of the spores will grow on YPD+G418)
> >
> > 2)  Possibly just a historical question, but, why does it seem that
> > antibiotic resistance
> > markers are used so seldomly in yeast work?
> >
> > Thanks for your help!
> >    Hui
> >     hcai at ucla.edu
> 
> --
> Krassen Dimitrov, Ph.D.
> Department of Molecular Biotechnology,
> Box 357730,
> University of Washington,
> Seattle, WA 98195
> phone: (206) 685-6892
> e-mail: krasen at u.washington.edu





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