pombe pfge

S L Forsburg nospamforsburg at salk.edu
Tue Nov 16 12:01:34 EST 1999

Jo Evans <j.evans at har.mrc.ac.uk> wrote
> Can anyone recomend a protocol for isolating the 3 pombe
> chromosomes within an agarose plug (for use in pulsed-field gel
> electrophoresis) I'm using the method in Nurses fission yeast hand
> book at the moment but would like to play around with a few more
> methods.

We use this method quite a bit; our protocols are published in Liang et
al (1999),    J. Cell Sci.  112:559-567 or Snaith and Forsburg, Genetics
 152: 839-851.

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S L Forsburg, PhD  forsburg at salk.edu
Molecular Biology and Virology Lab          
The Salk Institute, La Jolla CA 

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