HELP: Yeast 3-hybrid problem

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Fri Nov 19 05:52:48 EST 1999


Bernhard Mayr wrote:
> 
> Hi,
> 
> I'm trying to do a yeast 3-hybrid screen using Clontech's pBridge and
> pACT2 vectors. My problem is, that whatever vectors I transform the
> yeast always grows on -His media, even when I transform empty pBridge
> and pACT2 vectors and rise 3-AT to > 200mM. The yeast wt-phenotype is
> OK (tried CG1945 and Y190), I repeated my transformation with a
> complete new set of reagents and media, still the same result.
> Has anybody got an idea what's wrong ?
> 
> Bernhard Mayr
> The Salk Institute
> Peptide Biology Labs
> 10010 North Torrey Pine Road
> La Jolla, CA 92037
> USA
> 
> Fon +1 858 453 4100 ext 1176


Do you also observe lacZ activity?

Ricky




______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca





More information about the Yeast mailing list