HELP: Yeast 3-hybrid problem

Frederik Boernke boernke at
Fri Nov 19 05:52:48 EST 1999

Bernhard Mayr wrote:
> Hi,
> I'm trying to do a yeast 3-hybrid screen using Clontech's pBridge and
> pACT2 vectors. My problem is, that whatever vectors I transform the
> yeast always grows on -His media, even when I transform empty pBridge
> and pACT2 vectors and rise 3-AT to > 200mM. The yeast wt-phenotype is
> OK (tried CG1945 and Y190), I repeated my transformation with a
> complete new set of reagents and media, still the same result.
> Has anybody got an idea what's wrong ?
> Bernhard Mayr
> The Salk Institute
> Peptide Biology Labs
> 10010 North Torrey Pine Road
> La Jolla, CA 92037
> Fon +1 858 453 4100 ext 1176

Do you also observe lacZ activity?


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