Gene replacement w. Zeocin casette

[Björn]

Hi Netters,

I wonder if anyone used the Zeocin marker from invitrogen to
make gene replacement in S. cerevisiae using short flanking homology ?

The Zeocin casette contains the TEF1 promoter and the CYC1 terminator.
Will these regions of homology lower the efficiency of integration 
much ?

Grateful for any answer, 
Sincerely

Björn Johansson




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