Gene replacement w. Zeocin casette

Björn bjorn.johansson at
Fri Nov 26 15:25:16 EST 1999

Hi Netters,

I wonder if anyone used the Zeocin marker from invitrogen to
make gene replacement in S. cerevisiae using short flanking homology ?

The Zeocin casette contains the TEF1 promoter and the CYC1 terminator.
Will these regions of homology lower the efficiency of integration 
much ?

Grateful for any answer, 

Björn Johansson

YEAST bionet newsgroup see:
YEAST e-mail: messages sent to yeast at
subscribe: e-mail biosci-server at with: subscribe yeast
unsubscribe: e-mail biosci-server at with: unsubscribe yeast
YEAST on the WWW:
problems with the YEAST newsgroup? E-mail the moderator: francis at

More information about the Yeast mailing list