pichia and ammonium chloride

[Roberto Cappai]

Hi,

I was wondering if it is possible to use ammonium chloride as the 
nitrogen source for
Pichia pastoris expressions rather than ammonium sulfate.


I ask becasue we have 15N labeled NH4Cl  and we want labeled  protein for NMR.

Thanks.


Robert



Roberto Cappai, Ph.D.
Department of Pathology
University of Melbourne
Parkville, Victoria, 3052
AUSTRALIA

Phone: 61-3-9344 5882  (or 5868)
Fax:     61-3-9344 4004
r.cappai at pathology.unimelb.edu.au




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