yield from GAL-promoter

news at ulrik.uio.no news at ulrik.uio.no
Wed Sep 1 15:11:31 EST 1999


Hello!

I am using a  2m-plasmid with a GAL1,10 promoter to express an
heterologous protein in S.cerevisiae, and I would like to know what
level of expression to expect. Can't see my protein on SDS-PAGE of
lysates. Can anyone give me a clue (in fx percentage of total protein)
?

from thomas. moen at biotek.uio.no





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca




More information about the Yeast mailing list