yield from GAL-promoter

news at ulrik.uio.no news at ulrik.uio.no
Wed Sep 1 15:11:31 EST 1999


I am using a  2m-plasmid with a GAL1,10 promoter to express an
heterologous protein in S.cerevisiae, and I would like to know what
level of expression to expect. Can't see my protein on SDS-PAGE of
lysates. Can anyone give me a clue (in fx percentage of total protein)

from thomas. moen at biotek.uio.no

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