Quick PCR on yeast cells?

biosci-request at net.bio.net biosci-request at net.bio.net
Fri Sep 3 12:07:01 EST 1999


This has worked well for me.
David Jansma


Nucleic Acids Res 1995 Dec 11;23(23):4924-5

A rapid and reliable DNA preparation method for screening a large number of 
yeast clones
by polymerase chain reaction.

Ling M, Merante F, Robinson BH


From: biosci-request at net.bio.net, Klaus Lehnert <k.lehnert at auckland.ac.nz>
To: nobody at net.bio.net
Subject: Quick PCR on yeast cells?
Date: Fri, 3 Sep 1999 08:32:09 +1200

Hi folks,

I need to amplify a 600bp product from S. cerevisiae. AS this should be a
one-off, I'm not to keen to prep genomic DNA. Is there a simpler way, eg
using a colony straight away?

Appreaciate all advice


--
Dr. Klaus Lehnert
University of Auckland
School of Medicine



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