loading fluorescent dyes in yeast

Patrick Bradshaw bradshaw.23 at postbox.acs.ohio-state.edu
Wed Sep 29 02:42:55 EST 1999


Our lab as well as several other groups have been unable to load acetoxymethyl 
(AM) esters of fluorescent dyes such as Fura-2, Indo-1, and BCECF in 
Saccharomyces cerevisiae.  Does anyone know the reason for this? Are these 
dyes not getting into through the cell wall or do the cells lack the esterase 
to cleave and trap the dye inside the cell?

					Patrick Bradshaw





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