G418 selection

David Keszenman-Pereyra D.K-Pereyra at sheffield.ac.uk
Thu Apr 13 10:15:24 EST 2000


"can anyone tell me if it is possible to select for G418 resistant 
S.cerevisiae colonies on rich yeast medium containing 2% 
galactose and 1% raffinose? 
H. van Attikum"

We have plated yeast cells on SC-ura containing 2% galactose and 
G418 for integration of KNMX cassette and kept a URA3 plasmid.

David Keszenman-Pereyra
University of Sheffield
Department of Molecular Biology & Biotechnology   
---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca






More information about the Yeast mailing list