a beast ate my yeast, help!

Dan Riggs riggs at scar.utoronto.ca
Tue Aug 1 09:33:20 EST 2000

We have been conducting a protracted 2 hybrid screen and finally got a
positive signal by x-gal selection.  Unfortunately, the master plate is
contaminated by a fungus which grows much faster than does yeast (on
triple drop out medium).  The yeast strain we are using is YRG2 from
Stratagene, and its genotype doesn't suggest to me that one could expect
to plate it on some selection medium to rid the cells of the
contaminant.  Does anyone have any advice?


Dan Riggs


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