A copurified contaminant?

HASHIGUCHI Kazunari kaz at kingyo.zool.kyoto-u.ac.jp
Wed Aug 9 06:17:31 EST 2000


Hello,
I have purified the DNA binding protein from yeast crude and/or
nuclear extract. Gel shift assay was performed as a marker of
activity.  Although the predicted molecular mass of the protein
is 20-40 kDa, substantial amount of proteins, whose size are
60-80 kDa, were copurified during all of FPLC-purification proccess.
What are these proteins!
Please tell me about these suspicious proteins!

---------------------------------------------------------------
  HASHIGUCHI Kazunari
      Ph.D Student, Laboratory of Radiation Biology, 
         Graduate School of Science, Kyoto University, JAPAN.
       mailto:kaz at kingyo.zool.kyoto-u.ac.jp


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