ade mutant sequences

Midori Harris midori at genome.stanford.edu
Wed Aug 9 16:07:39 EST 2000


Hello,

Some of the ade2 alleles used as markers can be found in the table of
Commonly Used Auxotrophic Markers at SGD (although it doesn't have a
reference for the exact sequence of the ade2-101 allele). The URL is

 http://genome-www.stanford.edu/Saccharomyces/alleletable.html

To find in vitro assays, you could try searching PubMed for the enzymes
SAICAR synthase (Ade1p) and AIR carboxylase (Ade2p). The PubMed URL is

 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed

I hope this helps.

Regards,

Midori

*****************************************************
 Midori Harris, Ph.D., Database Curator
 Saccharomyces Genome Database
 Stanford University Medical Center
 Department of Genetics
 Stanford, CA 94305-5120 USA
 phone: (650)725-8956  fax: (650)723-7016
 e-mail:   yeast-curator at genome.stanford.edu
 WWW:   http://genome-www.stanford.edu/
*****************************************************

> 
> 
> ---------- Forwarded message ----------
> Date: 9 Aug 2000 02:09:31 +0100
> From: Linda Silveira <silveira at jasper.uor.edu>
> To: yeast at net.bio.net
> Newsgroups: bionet.molbio.yeast
> Subject: ade mutant sequences
> 
> Does anyone know the exact sequence alterations in any common
> mutant alleles of ade1 and ade2 (e.g., ade2-101)?
> 
> Also, is there an (easy?) in vitro enzyme assay for either Ade1p or Ade2p?
> 
> Thanks,
> 
> Linda Silveira
> 
> Associate Professor
> University of Redlands
> 1200 E. Colton Ave.
> Redlands CA 92373-0999
> 
> silveira at uor.edu
> 
> 
> 
> ---
> 
> 
> 


---





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