Could be yeast still transformed by DNA after treatment of EMS?

Jason Harger hargerja at UMDNJ.EDU
Thu Feb 3 15:24:54 EST 2000


I would like to mutagenize yeast and screen the mutants that are
resistant to ribosome toxin expressed by a plasmid. I prefer not to put
the plasmid into yeast before treatment of EMS since I will potentially
get a lot plasmid bourne mutants. But I am not sure if yeast after
treatment of EMS could take DNA by LiOAC transformation. Appreciate any
suggestion.

Peter

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