thiamine question

Long, Kimberly Kimberly.Long at umassmed.edu
Thu Feb 3 17:09:08 EST 2000


Hi,

I am a new graduate student working with S. pombe.  My favorite gene is
under the control of the nmt1 promoter and I am doing a repression
experiment over a long time course.  Can anyone tell me how long thiamine
lasts in media at 30 C?  Do I need to replenish the thiamine after a certain
amount of time?

Thank you,
Kim

............................................................................
.................................................
Kimberly R Long
Dept. of Molecular Genetics & Microbiology
UMass Medical School
Kimberly.Long at umassmed.edu
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