sex on a plate

darren.wells at darren.wells at
Tue Jan 18 07:25:05 EST 2000

Dear All,
I am wanting to combine two Saccharomyces cerevisiae strains of opposite
mating type.  The first has a gene inserted into its genome which I am
intressted in.  The second in a knockout of a different gene which my
supply a good background to test the activity of the inserted gene.
Does anyone have a protocol for bringing these two together.  My
knowledge and experience of yeast is limited so something simple would
be a great help.
The two strains are;
his 3delta1; leu 2delta0; met15delta0; ura 3delta0
leu 2-3; leu2-112; deltatrp1; ura3-52

I think that a diploid may be selected via the trp and met autotrophies
Thanks for your help.

nick.cryer at


YEAST bionet newsgroup see:
YEAST e-mail: messages sent to yeast at
subscribe: e-mail biosci-server at with: subscribe yeast
unsubscribe: e-mail biosci-server at with: unsubscribe yeast
YEAST on the WWW:
problems with the YEAST newsgroup? E-mail the moderator: francis at

More information about the Yeast mailing list