Two-hybrid system for proteins localised in the vacuole

Esbjorn Fiers esfie at gengenp.rug.ac.be
Fri Jan 21 05:28:28 EST 2000


Hey David,

it is certainly true that the conditions in those cell compartments are
different from conditions in the nucleus. For example, in the golgi (secretory
pathway) the redox potential is more oxidizing then in the cytosol and the
nucleus. Therefore, disulfide bridges will not be formed in the nucleus and
proteins with structural disulfide bridges (as a lot of receptors are) will not
interact with there native ligands in two-hybrid format.

Hope this helps you out a little bit,

Esbjorn Fiers

ps: maybe the next articles are also of interest to you:

      Ozenberger, et al. Functional interaction of ligands and receptors of
the hematopoietic superfamily in yeast. Mol Endocrinol 1995;9:1321-9.

      Ozenberger, et al. Investigation of ligand/receptor interaction and the
formation of tertiary complexes. In: Bartel P, Fields S (eds.), The Yeast
Two-Hybrid System. New York: Oxford University Press; 1997; 158-72.




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Esbjorn Fiers
DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Vlaams Instituut voor Biotechnologie                           VIB
mailto:esfie at gengenp.rug.ac.be        http://www.plantgenetics.rug.ac.be
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