zhaojun at public.wh.hb.cn
Sun Jul 23 05:38:33 EST 2000
** Note from the moderator:
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** For this one, maybe something like:
** "need help with cloning ds-oligo"
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** cheers, bffo
We try to clone a the double stranded Oligos ( with Hind III and XbaI at each end of the Oligo) into pcDNA3 vector, but I don't know whether the Oligos should be phosphated? If yes, would you please provide a detail protocol for phosphorylation using T4 polynucleotide kinase? In addition, what kind of ATP should be used in the reaction?
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