Changing Markers

Tiago FLeming tfdeoliv at midway.uchicago.edu
Fri Jun 2 09:23:38 EST 2000


My name is Tiago Outeiro.

I have a construct in a pRS306 vector, which as you might know has a URA
marker for selection. It happens that I cannot use this marker for the
purpose of my work, and I would like to know of an easy and fast way for
replacing that marker with a LEU or HIS, without involving cloning.
Can anyone help?

Thanks in advance,

Tiago



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