Yeast colony PCR - who can do it?

Joerg Schenk jschenk at
Thu Jun 8 07:52:40 EST 2000

Hi Thomas,
some years ago we were successful in amplifying some fragments from yeast strain
HF7c without isolating the plasmids. Maybe the initial step at 94°C has to be
longer than usual then. You can have a look at :
Biotechniques 20: 812-816 (1996).
Recently, there was another paper by Akada where they isolated the
plasmids by simply put the colony into 0.25% SDS, mixing and put 1µl to the PCR
reaction. By adding Triton the results were even better (Biotechniques 28:
668-74, (2000)).
Hope this helps



YEAST bionet newsgroup see:
YEAST e-mail: messages sent to yeast at
subscribe: e-mail biosci-server at with: subscribe yeast
unsubscribe: e-mail biosci-server at with: unsubscribe yeast
YEAST on the WWW:
problems with the YEAST newsgroup? E-mail the moderator: francis at

More information about the Yeast mailing list