Yeast colony PCR - who can do it?

Joerg Schenk jschenk at mdc-berlin.de
Thu Jun 8 07:52:40 EST 2000


Hi Thomas,
some years ago we were successful in amplifying some fragments from yeast strain
HF7c without isolating the plasmids. Maybe the initial step at 94°C has to be
longer than usual then. You can have a look at :
Biotechniques 20: 812-816 (1996).
Recently, there was another paper by Akada et.al. where they isolated the
plasmids by simply put the colony into 0.25% SDS, mixing and put 1µl to the PCR
reaction. By adding Triton the results were even better (Biotechniques 28:
668-74, (2000)).
Hope this helps

Joerg

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