Gurumurthy D S
gmurthy at tifr.res.in
Mon Jun 12 04:05:44 EST 2000
I need some help with DNA mobility shift expts. I have seen that
one of the components of the reaction mixture is poly dI-dC. I would like
to know a few things about it.
1. It it a repeat of dI-dC like ICICICICICIC......?
2. How long is the polymer that is used in expts?
3. Does the polymer has to be a double stranded or single stranded
4. Can a polymer synthesized be used directly in the expts or is
there any pretreatments to be done before use?
Any help in these regards will be highly appreciated.
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