bhoopes at mail.colgate.edu
Mon Jun 12 09:19:58 EST 2000
Although I don't know how long the polymer is, it is a repeat of
ICICetc. Whether or not single stranded will do depends on the DNA binding
proteins you are trying to compete. No pretreatment was required in our
hands (we were using it in TBP bandshifts, where it functions as a specific
competitor, since ICIC looks like TATA in the minor groove).
> From: Gurumurthy D S
> Sent: Monday, June 12, 2000 5:05 AM
> To: yeast at net.bio.net
> Subject: poly dI-dC
> I need some help with DNA mobility shift expts. I have seen that
> one of the components of the reaction mixture is poly dI-dC. I would like
> to know a few things about it.
> 1. It it a repeat of dI-dC like ICICICICICIC......?
> 2. How long is the polymer that is used in expts?
> 3. Does the polymer has to be a double stranded or single stranded
> will do?
> 4. Can a polymer synthesized be used directly in the expts or is
> there any pretreatments to be done before use?
> Any help in these regards will be highly appreciated.
> Thank you
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