Gap repair

Hans-Peter Vornlocher vornlocher at
Thu Mar 2 11:06:48 EST 2000


who can provide a working protocol for a gap repair procedure in S.cerevisiae?
I am trying to create a library of a mutagenized protein for two hybrid
screening by cotransforming PCR product and a gapped plasmid. How many
nucleotides overlap between PCR-product ends and linearized vector is




Dr. Hans-Peter Vornlocher
Institut für Molekularbiologie und Tumorforschung
Emil-Mannkopff-Straße 2
Philipps Universität Marburg
D-35033 Marburg

Tel.: 	++ 06421/2865325
FAX:	++ 06421/2867008
e-mail:	vornlocher at


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