Gap repair

Hans-Peter Vornlocher vornlocher at imt.uni-marburg.de
Thu Mar 2 11:06:48 EST 2000


Hello,

who can provide a working protocol for a gap repair procedure in S.cerevisiae?
I am trying to create a library of a mutagenized protein for two hybrid
screening by cotransforming PCR product and a gapped plasmid. How many
nucleotides overlap between PCR-product ends and linearized vector is
required?

Thanks,

Hans-Peter



*************************************************

Dr. Hans-Peter Vornlocher
Institut für Molekularbiologie und Tumorforschung
Emil-Mannkopff-Straße 2
Philipps Universität Marburg
D-35033 Marburg

Tel.: 	++ 06421/2865325
FAX:	++ 06421/2867008
e-mail:	vornlocher at imt.uni-marburg.de

*************************************************
---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca






More information about the Yeast mailing list