Yeast Expression Vectors

Younes Bounhar ybounh at
Tue Mar 14 19:48:43 EST 2000

I have a construct with over 100bp of 5' DNA and it expresses just fine on a
Gal -inducible vector. I heard though that you should check if your 5'
sequence has a lot of secondary structure (eg loops)

James Harper <jah49 at> wrote in message
news:8alhfu$85l$1 at
> Dear Yeast People,
> I'm using two different galactose inducible vectors (pYES2 and pKV49).
> I was wondering if anybody knows if the length of the untranslated
> region between the Gal promoter and ATG is related to the level of
> protein expression.
> Any information gladly received.
> James


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