Quick, simple way to purify ADH?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Mon Mar 20 07:39:12 EST 2000


I am thinking of introducing the purification of yeast ADh into a student
lab class. They already fractionate yeast to localise ADH, then do SDSPAGE,
gel filtration on commercial prep to characterise it.  It would be nice if
they could at least semi-purify it.  So waht I'm after is a simple
procedure.  How about ammonlium sulphate ppt of cytosol followed by
affinity step?  I know NAD analogues can be used as affinity matrices, but
how about ethanol (coupling?) or an analogue?  Any advice appreciated.

-- 
Fergus Doherty,
School of Biomedical Sciences,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)
---





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