Extraction of plasmid DNA from yeast

Colin Rasmussen colin at schizo.usask.ca
Mon Mar 20 15:24:40 EST 2000



> From: Ashok Aiyar <a-aiyar at nwu.edu>
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Newsgroups: bionet.molbio.yeast
> Date: 19 Mar 2000 03:15:31 -0000
> Subject: Extraction of plasmid DNA from yeast
> 
> 
> We extract plasmid DNA from yeast by vortexing in the presence
> of acid-washed glass beads, Triton X-100, SDS, and phenol-chloroform.
> 
> This method has never worked particularly well for me, and we are
> close to running out a batch of acid-washed beads that was gifted to
> me several years ago.
> 
> So I have two questions:
> a) what grade glass-beads should I buy, and how do I acid-wash them?
> b) Are there any alternative methods that work more efficiently and
> reproducibly?

We buy beads from Sigma and we don't bother doing anything special to them.

Colin Rasmussen

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