Two-hybrid strains

Ricardo Azpiroz razpiroz at hotmail.com
Thu Mar 30 18:27:31 EST 2000


I'm looking for a strain called PJ69-4A, or something similar in the sense 
of having a GAL-controlled ADE2 gene. This strain sounds particularly nice 
for 2-hybrid screening because most markers are non-reverting. I'm not 
really interested in the GAL-HIS3 thing, since I hear it leaks false 
positives like crazy, maybe because that his3delta200 is that promoter 
deletion that has some basal transcription (?).

Also, if anybody can spare a few molecules of pNKY51 I would greatly 
appreciate it.

I'm at a little startup biotech company, trying to figure out what works 
best in our hands. Thanks for the help.


Ricardo Azpiroz
Hybrigen, Inc.
Dallas, TX
USA
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca






More information about the Yeast mailing list