GFP tags in yeast
grant at zoology.ubc.ca
Fri Mar 31 17:09:32 EST 2000
On a similar note. Does anybody have a Saccharomyces strain with GFP
integrated into the genome? Are you willing to lend it out?
On 31 Mar 2000, B.Drees wrote:
> I am thinking about tagging some yeast proteins with GFP or GFP variants
> for localization. I'm trying to decide on the best strategy to use for
> this. My main questions are:
> - Integrate tag into genome or express fusion on plasmid vector? So far
> my integrated tags are invisible so I am leaning toward using plasmids.
> - N-terminal or C-terminal fusions? Will an N-terminal fusion screw up
> localization signals?
> - Inducible or constitutive promoter on the vector?
> Becky Drees
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