two-hybrid vector for N-terminal fusions to GAL4-DNA-BD

Barry Young byoung at plato.wadham.ox.ac.uk
Tue May 23 12:22:19 EST 2000


On 23 May 2000 15:47:37 +0100, jean-claude jauniaux 
<J.Jauniaux at dkfz-heidelberg.de> wrote:
>Dear all,
>
>I am looking for a yeast two-hybrid vector which produces N-terminal fusion
>to GAL4 DNA-BD domain, with preferentially TRP1 as selective marker
>Thank you for your help


Not quite, but I was rummaging through my reference list the other day and
stumbled across:

"Vectors for Expressing Proteins at the Amino-Terminus of an Activation Domain 
 for Use in the Yeast Twi-Hybrid System" 

Brown, M.A. and MacGillivray, R.T. (1997) Analyt.Biochem. 247:451-452

Wrong domain, but it might be of some use to you. You never know, they might 
have a  BD equivalent. M.A. Brown's e-mail address is mabrown at unixg.ubc.ca


Barry

-- 
Barry Young
School of Biological Sciences
University of Manchester
Manchester
UK
---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca






More information about the Yeast mailing list