retesting interaction

nakada nakada at
Mon Nov 6 00:46:10 EST 2000

Hello, everyone.

I'm working with yeast-two hybrid system. I have a questin about
retesting interaction between my bait protein and play protein found
in a library. I have done library screeninng and got some clones those
ware supposed to interact with my bait rotein. I tried to confirm those
interactions by cotransformation of the bait and play plasmid,
I could not get any colonies on SD selection medium plates, however.
Dose this occure offen in yeast two hybrid system ? I would greatly
apreciate if
you give me suggestions or comments.

Genox Research, Inc.
Setagaya Lab.
e-mail; nakada at


YEAST bionet newsgroup see:
YEAST e-mail: messages sent to yeast at
subscribe: e-mail biosci-server at with: subscribe yeast
unsubscribe: e-mail biosci-server at with: unsubscribe yeast
YEAST on the WWW:
problems with the YEAST newsgroup? E-mail the moderator: francis at

More information about the Yeast mailing list