pBridge

Ralf Weigel weigeler at uni-hohenheim.de
Wed Nov 15 10:18:12 EST 2000


Dear Kazuo,

we have used the pBridge vector quite successfully with the strains HF7c
and SFY526 on SD lacking methionine. If your strain does not have a
mutation in its methionine pathway it should grow since all none
essential amino acids should be metabolized. I propose: try a different
strain.

    Ralf

--

Ralf R. Weigel

Albrecht von Haller Institute of Plant Sciences
General and Developmental Plant Physiology
Untere Karspuele 2
37073 Goettingen

Tel.:  0551/3919664
Fax: 0551/397820



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