help with invertase assay

Valmik K.Vyas valmik at alum.mit.edu
Thu Nov 23 10:28:58 EST 2000


Hi Alex,
    From what I have heard, Pfanstiehl sucrose is the best in terms of low
glucose contamination. Other brands (like Sigma) have a pretty high glucose
content.   This is what my lab uses for invertase assays (I'm a grad student in
Marian Carlson's lab).  I hope this helps.
take care,
valmik

Alex Brands wrote:

> Hello all,
>
> I am trying to do invertase assays in lab, but I'm having a problem with
> the sucrose.  The problem is that the sucrose is contaminated with
> glucose.  Since the assay is suposed to measure how much glucose is
> liberated from sucrose, this is a big problem as it gives me a very high
> background signal.  I tried sucrose from other labs, and they all have the
> same problem.
>
> Can anyone tell me what is the best way to make a glucose free sucrose
> solution?  My first option right now is to treat the sucrose solution with
> glucose oxidase and catalase, but I'm hoping there is a simpler way.
>
> Thanks!
>
> Alex Brands
> Washington University
>





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