prey - bait ??

bonyadi bonyadi at aurore.cict.fr
Sat Nov 25 08:57:44 EST 2000


Hi every body,
I used my bait to find its partners throughout the library.
I found couple of them.
One question:
I have a prey (cDNA), which was initially positive. Now this prey is acting
strange ? 
well, When I perform co-transformatio of this prey + plasmid (which has the
bait), i don`t see any colony in Selective media.
However, the interesting part is that, when I performe co-transformatioin of
this plasmid (prey) + the plasmid (without the bait = empty plasmid) , I am
getting very good colonies in selective media.

I am using AH109 strain, and the selective media is -Ade -Tr -Leu -His.
* If it is autoactivation (of prey): I have to see colonies in both cases???

Any suggestion would be apreciated,
Morteza,
-------------------------------------------------------------------
Morteza Bonyadi, PhD,
Lab. De Biologie Cellulaire Et Moleculaire Du Controle De La Proliferation,
Universite Paul Sabatier,
CNRS UMR 5088- Batiment 4R3 B1,- 118 rte de Narbonne-
31062 TOULOUSE CEDEX 4,
France
Email: 
Bonyadi at cict.fr
Bonyadijm at yahoo.com
Bonyadim at tbzmed.ac.ir
Tel/Fax : ++33 5 61 55 69 19






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