AD fusion Library
pickuen at hotmail.com
Tue Oct 3 05:04:07 EST 2000
I am working with the yeast one hybrid system. I have already get 80% of my
reporter strain done. My biggest problem is that I still don't have an AD
fusion library. I have tried to clone it into pGAD424 using commercial
chemical competent cells and electroporate into pPC86 but both have
tremendously fail. I have tried the Hybrizap from Stratagene but the test
insert from the kit has only 3000pfu/ml.
Is there any suggestion?
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