gap repair

Ralf Weigel weigeler at uni-hohenheim.de
Fri Oct 6 10:49:09 EST 2000


Dear colleagues,

I have a question concerning the gap repair process in yeast. Why does
transformation of  a gapped plasmid alone (negative control) also lead
to growth of colonies (with low efficiency) although the plasmids ends
are incompatible.  Is the plasmid somehow recircularized or even
integrated into the yeast genome ?
Any suggestions or comments are welcome.

    Ralf

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Ralf R. Weigel

Albrecht von Haller Institute of Plant Sciences
General and Developmental Plant Physiology
Untere Karspuele 2
37073 Goettingen

Tel.:  0551/3919664
Fax: 0551/397820



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