gap repair

Ralf Weigel weigeler at
Fri Oct 6 10:49:09 EST 2000

Dear colleagues,

I have a question concerning the gap repair process in yeast. Why does
transformation of  a gapped plasmid alone (negative control) also lead
to growth of colonies (with low efficiency) although the plasmids ends
are incompatible.  Is the plasmid somehow recircularized or even
integrated into the yeast genome ?
Any suggestions or comments are welcome.



Ralf R. Weigel

Albrecht von Haller Institute of Plant Sciences
General and Developmental Plant Physiology
Untere Karspuele 2
37073 Goettingen

Tel.:  0551/3919664
Fax: 0551/397820


YEAST bionet newsgroup see:
YEAST e-mail: messages sent to yeast at
subscribe: e-mail biosci-server at with: subscribe yeast
unsubscribe: e-mail biosci-server at with: unsubscribe yeast
YEAST on the WWW:
problems with the YEAST newsgroup? E-mail the moderator: francis at

More information about the Yeast mailing list