quantitative lacZ measurements

Jackson, John C. JohnC.Jackson at anheuser-busch.com
Wed Sep 13 06:55:44 EST 2000


For a simple ONPG assay that works incredibly well and reproducibly, try the
Yeast B-gal kit from Pierce Chemical (www.piercenet.com) or use the CLONTECH
protocol in their Yeast Protocols Manual (www.clontech.com).  You could also try
using CPRG as a substrate, which is supposed to be more sensitive than ONPG,
although it is more expensive and in my experience, more of a pain to deal with
than ONPG (again, the CLONTECH manual has a protocol for this).  Of course, for
ultimate sensitivity, try a chemiluminescent b-gal assay.  Tropix
(www.tropix.com) has a chemiluminescent yeast b-gal kit.  Hope these help.

--John


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