32P labeling of S. Cerevisiae

L.Weisman lweisman at blue.weeg.uiowa.edu
Wed Sep 13 10:39:59 EST 2000


My laboratory has achieved excellent incorporation of 32P following
the protocol in Rubin, G. 1976. Methods in Cell Biology 12:45-64.  The
phosphate depleted media described in that paper is made by selective
precipitation of inorganic phosophate (as MgNH4PO4) from YEPD.  

On Tue, 12 Sep 2000, Brian Baker wrote:

> I'm having a hard time trying to label my yeast cells with 32P. the
> label does not seem to be incorporated. I'm using 32P in the form of
> H3PO4, I'm growing my cells in -phosphate media, and I label them for
> ~45 min at 30 C. Does anyone know what's I can do? or have a better
> protocol?
> 
> 

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