YPD medium for cerevisiae

Alex Brands abbrands at artsci.wustl.edu
Mon Sep 18 13:32:54 EST 2000


On 18 Sep 2000, Hashiguchi Kazunari wrote:

> When you make the YPD medium, do you autoclave "polypeptone + yeast
> extract + glucose" in one bottle?
> At the same time, the medium is colored with dark brown.
> I should autoclave only the glucose, independently?

I usually autoclave them together.  I usually do a 20 minute cycle and
take the media out as soon as the pressure comes down.  If I leave it in
too long, it does turn progressively more brown.

If you autoclave the glucose solution seperately, you will avoid
this phenomenon.

Alex


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