YPD medium for cerevisiae
abbrands at artsci.wustl.edu
Mon Sep 18 13:32:54 EST 2000
On 18 Sep 2000, Hashiguchi Kazunari wrote:
> When you make the YPD medium, do you autoclave "polypeptone + yeast
> extract + glucose" in one bottle?
> At the same time, the medium is colored with dark brown.
> I should autoclave only the glucose, independently?
I usually autoclave them together. I usually do a 20 minute cycle and
take the media out as soon as the pressure comes down. If I leave it in
too long, it does turn progressively more brown.
If you autoclave the glucose solution seperately, you will avoid
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