Beta galactosidase assay

chan pickuen pickuen at hotmail.com
Tue Sep 26 23:15:15 EST 2000


Hi,

I am working with the yeast one hybrid (the kit is from Clontech). I am 
trying to construct lacZ reporter yeast strain. I have to screen for 
transformants using the beta Galactosidase assay via the colony lift method. 
I don't seems to have any positives from test. The positive control from the 
kit also does not show any color change. I have tried the protocol from 
Current Protocol and also protocol from Clontech. Could it be the low 
expression? I intend to use the strain for my screening.

Chan Pickuen
Universiti Putra Malaysia

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