Kozak sequences and yeast

[Dr.Duncan Clark]

Hi Folks,

I am having to get involved with yeast expression and am not sure if I
need both an rbs and a kozak sequence. We are planning to use the
Invitrogen vector pYC6/CT but need to ensure that our gene starts with
it's own ATG. The vector only has a GAL1 promoter so we presume we must
design in the kozak sequence. Do we therefore need another rbs? I'm so
used to expression in E.coli that I need to touch base on yeast.

Many thanks

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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