hot PCR

Monica Montero Lomeli MONTERO at
Wed Jun 20 14:28:01 EST 2001

I would like to perform a PCR using radioactive 32P alpha-dCTP and 
then use the product as a probe for Northern Blot. Does any body have 
a protocol for the Hot PCR?
Thanks in advance,
Monica Montero-Lomeli
Universidade Federal do Rio de Janeiro
Departamento de Bioquimica Medica
ICB-CCS CP 68041, Rio de Janeiro
RJ 21941-590, Brasil
Tel. (55-21)270-1635
Fax. (55-21)270-8647


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