Background info on yeast transformation

Alex Brands abbrands at artsci.wustl.edu
Fri Jun 29 10:42:52 EST 2001


On 29 Jun 2001, Cathy Slack wrote:
> I have recently started working with yeast and was wondering if there is
> somewhere I can all the background info on yeast transformations. The
> stuff i am looking for is:
>
> Salmon sperm acts as a carrier...but how and what happens to it after
> the transformation?

Some of it will be stuck to the yeast cells, some will be washed away with
the PEG.

> What does the PEG do??

The PEG precipitates the DNA, hopefully onto the yeast cells.

> What does DMSO do??

I believe it helps to permeabilize (sp?) the cells.


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