Background info on yeast transformation
abbrands at artsci.wustl.edu
Fri Jun 29 10:42:52 EST 2001
On 29 Jun 2001, Cathy Slack wrote:
> I have recently started working with yeast and was wondering if there is
> somewhere I can all the background info on yeast transformations. The
> stuff i am looking for is:
> Salmon sperm acts as a carrier...but how and what happens to it after
> the transformation?
Some of it will be stuck to the yeast cells, some will be washed away with
> What does the PEG do??
The PEG precipitates the DNA, hopefully onto the yeast cells.
> What does DMSO do??
I believe it helps to permeabilize (sp?) the cells.
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