confirming yeast 2-hybrid positives / X-gal assay

Alex Brands abbrands at artsci.wustl.edu
Wed Mar 28 11:23:47 EST 2001


On 26 Mar 2001, Weiwei Liu wrote:
> I extracted plasmids from my positives and transformed them back into the bait
> strain, but when I assayed the back-transformants, none of my 22 positive
> clones yielded blue color in the X-gal filter lift assay. Does this mean that
> they're all false-positives? (Oh, I hope not!)
>
> And, if there's something wrong with the X-gal assay, what is the most
> possible reason for it?

Do you have a positive control for the X-gal assay?



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