why no insert from blue colonies
dongzha at yahoo.com
Sun Apr 14 22:53:07 EST 2002
I need help on yeast two-hybrid library screening.
I got many positive (blue) colonies using clontech
system 3. The colonies look so blue on
-Trp-Leu-His-Ade plates with X-alpha-gal.
I pick up 100 colonies to do colony PCR for amplify
insert on AD-vector.
The problem is that only, only 40% have shown insert.
Why not another 60% percent? Are they false positive?
If there is no AD vector at all, how could these
colonies grow on the selection plate?
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